显著性检验通常可以告诉我们一个观测值是否是有效的,例如检测两组样本均值差异的假设检验可以告诉我们这两组样本的均值是否相等(或者那个均值更大)。我们在实验中经常会因为各种问题(时间、经费、人力、物力)得...
Tophat中-r/–mate-inner-dist参数
随着测序技术的蓬勃发展,转录组测序中Tophat的应用愈来愈频繁。但是很多论坛,博客等,都在针对Tophat中-r/--mate-inner-dist参数的解释不禁让我觉得有些不妥。 版本一: -r ...
Testing SOAPdenovo2 Prerelease – V (map and scaff)
We would like to complete our description of SOAPdenovo2 commands and get into more interesting topi...
Testing SOAPdenovo2 Prerelease – IV
SOAPdenovo2 runs through the following steps - (i) generate the de Bruijn graph and store it (‘pregr...
Testing SOAPdenovo2 Prelease Version – III
This commentary is a continuation of previous one. We are adding some additional information in case...
Testing SOAPdenovo2 Pre-release Version II:pregraph_sparse
Our earlier commentary on SOAPdenovo2 provided a quick overview of an assembly. In the next few comm...
Testing SOAPdenovo2 Pre-release Version
Opening the zip file: The zip file ‘SOAPdenovo2.zip’ expanded into two programs - i) pregraph_sparse...
Howto make N50 better?
N50 statistic is widely used by bioinformaticians to judge the quality of their assemblies, but it i...
RNASeq-MATS:Multivariate Analysis of Transcript Splicing
给大家推荐一款最新在Nucleic Acids Research上发表的基于转录组数据分析可变剪接的软件。下面是官方网扎你上的介绍。 MATS is a computational tool to d...
在de Bruijn Graphs中利用mate pair的信息
This is the last post in the de Bruijn graph series. Although we did not mention explicitly, each of...