GenomicsPacific Bio用于基因组组装 We are really amateurs, when it comes to Pacific Bio sequences. How amateur are we? A few weeks back... 06月24日2,132评论基因组组装 序列 阅读全文
Glossary基因组拼接中常见的名词解释 Read:高通量测序平台产生的序列就称为reads。 Contig:拼接软件基于reads之间的overlap区,拼接获得的序列称为Contig(重叠群)。 Scaffold:基因组de novo测序... 06月10日10,0395NGS 测序 阅读全文
GenomicsNewbler output II: contigs and scaffolds sequence files, and the 454Scaffolds.txt file The files most people are after when they do an assembly must be these: the actual contig and scaffo... 03月30日1,466评论454 拼接 阅读全文
GlossaryWhat is scaffold? A scaffold is a portion of the genome sequence reconstructed from end-sequenced whole-genome shotgun... 12月18日2,0481 scaffold 阅读全文
GenomicsSSPACE:一款专门做scaffolding的软件 在做基因组拼接的时候,有时候已经获得了一堆contig或者scaffold,然后需要利用另外的一个pair-end或者mate-pair文库进一步做scaffolding的话。用什么软件好呢? 最近使... 10月30日6,84412assembly genome 阅读全文